Journal: Biomedical Optics Express
Article Title: Hollow core photonic crystal fiber for monitoring leukemia cells using surface enhanced Raman scattering (SERS)
doi: 10.1364/BOE.6.004599
Figure Lengend Snippet: Number of events vs. total number of cells/mL for HL60 cells. Number of events was quantified by using the scattering upon 488-nm excitation of a non-stained cell suspension. Top inset shows the scattering profile for the sample containing 25,000 cells/mL. Bottom inset shows a magnification for the lower cell numbers where the red area denotes the noise region or lower limit of detection for the system. Right panels include the scattering profile for the sample with 310 cells/mL and a control solution without any cells, where it can be clearly seen the close similarity between the two plots.
Article Snippet: 2.2 Sample preparation Acute promyelocytic leukemia (HL60) cells (ATCC® CCL-240) were cultured in Iscove's Modified Dulbecco's Medium (Sigma), supplemented with 20% fetal bovine serum and 1% antibiotics (streptomycin and penicillin) and 0.1% gentamicin.
Techniques: Staining, Suspension, Control